Study on the therapeutic efficacy of subcutaneous pancreatic cancer xenograft tumor with ultrasound destruction of different size microbubbles

Abstract

Objective To assess the therapeutic efficacy of subcutaneous transplantation pancreatic cancer in nude mice model with different size microbubbles. Methods Eighteen Balb/C nude mice with subcutaneous pancreatic carcinoma xenografts were divided into three groups: group A(6 mice) did not received microbubbles and ultrasonic irradiation; group B(6 mice) received standard microbubbles and ultrasonic cavitation treatment; group C(6 mice) received up-sized microbubbles and ultrasonic cavitation treatment. Body weight and tumor size of nude mice were measured respectively at the time points of 0, 7, 14, 21 days after three times ultrasound radiation and before ultrasound treatment. Tumor volumes were calculated.Then the mice were sacrificed finally. Results There was no significant difference in tumor volumes among three groups before treatment(P>0.05). Tumor volume continued to increase in all groups. Compared with group A, growth rate in group B and group C were lower, growth rate in group B was higher than that in group C, the difference was statistically significant(P 0.05). RBV and RBF were decreased significantly after 3 days treatment in group B and group C(P 0.05). Conclusions The cavitation effect of up-sized microbubbles is more efficient for mice subcutaneous pancreatic cancer xenograft tumor inhibition compared with standard microbubbles. Key words: Sonication; Pancreatic neoplasms; Microbubbles; Neoplasms, vascular tissue