Abstract

The influence of oil rate and quality of rice bran oil by enzymatic extraction ultrasonic assisted were studied through designed experiment. The optimum enzymatic hydrolysis conditions were determined by the way of the orthogonal test. The results showed: after lipase enzyme in the rice bran (40 mesh sieve) was killed by the hot steam, the solution compounding material liquid ratio was 1:5 (W/V), 60°C, power and time of ultrasonic treatment were 120 W and 55 min, cellulose 1.2%, protease 0.6% and amylase 0.3%, pH 4.5, temperature 55°C, enzyme hydrolysis 5.5 h, the oil rate would reach to 92.63%.

Highlights

  • Rice bran is the by-product of rice processing, accounted for 5-8% in grain weight

  • The tests were conducted to study the influence of rice bran oil rate on the time, power and temperature of the ultrasonic treatment

  • Time of ultrasonic treatment: The results showed, in the range of ultrasonic treatment time 30-50 min, rice bran oil rate increase rapidly with the treatment time

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Summary

Introduction

Rice bran is the by-product of rice processing, accounted for 5-8% in grain weight. Rice bran contains grease (12-24%), protein (12-15%), cellulose (9.810%), vitamins (VB1, VB2, VP) and mineral elements. Rice bran oil, extracted from the rice bran, contains the unsaturated fatty acids, accounted for 38-42% oleic acid and 32-35% linoleic acid. The linoleic acid is widely recognized as an essential fatty acid and decreased the blood cholesterol, prevents atherosclerosis and other health effects, so the rice bran oil is a kind of ideal edible vegetable oil (Wang, 2006; Marlene et al, 2005). Compared with other preparation process, enzymatic extraction can destroy cell wall of rice bran by the hydrolysis of cellulase, protease and amylase, so that fat within the cell wall release as possible, mild reaction condition, simple equipment, high rate and quality of rice bran oil (Ma et al, 2005; Aparna et al, 2001). Raw material pretreatment and enzymatic hydrolysis conditions by ultrasonic enzymatic extraction of Timmy bran oil were studied and the extraction process parameters were optimized through the orthogonal experiment

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