Study on the Optimization of Extraction Technology of Anemonin from &amp;lt;i&amp;gt;Pulsatilla chinensis&amp;lt;/i&amp;gt; and Its Inhibitory Effect on &amp;lt;i&amp;gt;Alternaria panax&amp;lt;/i&amp;gt;

Xue Fang,Jinlei Liu,Lirui Yang,Xiangguo Li

doi:10.11648/j.jdmp.20190506.12

Study on the Optimization of Extraction Technology of Anemonin from &lt;i&gt;Pulsatilla chinensis&lt;/i&gt; and Its Inhibitory Effect on &lt;i&gt;Alternaria panax&lt;/i&gt;

Xue Fang, Jinlei Liu + Show 2 more

Open Access

https://doi.org/10.11648/j.jdmp.20190506.12

Copy DOI

Abstract

Background: The main antibacterial component of Pulsatilla chinensis is anemonin, which has strong antibacterial effect, so it is studied. The Pulsatilla chinensis is one of the diseases that are difficult to solve in the growth of ginseng, but chemical control not only causes harm to the environment, but also has residues on ginseng itself. Pulsatilla is a good medicinal plant. It will have a certain effect on the prevention and treatment of ginseng disease, and it can do no residue, no harm to ginseng. Objective: This experiment can provide effective data support for the prevention and treatment of Pulsatilla chinensis. Method: In this experiment, Pulsatilla Chinensis was used as experimental materials to extract plant by reflux, ultrasonic extraction methods, the content of anemonin in each single factor experiment was determined by HPLC. According to the content, the response surface analysis method was used to find the best extraction process, and the mycelium growth rate method was further used to determine the antifungal effect of Alternaria panax. Results: The EC50 value of P. Chinensis extract to A. panax was methanol extract < acetone extract < ethanol extract in order from small to large; The optimum extraction conditions of anemonin, the effective component of P. Chinensis methanol extract, were as follows: methanol volume fraction 87%, extraction time 2 h, solid-liquid ratio 20 (V: m), the optimum extraction amount was 3.760 μg·g-1.

Highlights

Pulsatilla chinensis (Bunge) Regel, ranunculaceae perennial herbs have the effect of clearing heat, detoxifying, cooling blood and stopping dysentery
Under the action of enzymatic hydrolysis, Ranunculin is decomposed into proto-Pulsatillarin, which is rapidly polymerized into dimer Pulsatillarin in air; at the same time, the main antimicrobial components of Pulsatilla chinensis are protoanemonin and anemonin [4,5,6], which have strong antimicrobial activity [7]
This method has the advantages of simplicity and high sensitivity, and through the verification test of the best extraction process, it is found that it is close to the theoretical extraction value, which indicates that the extraction amount can be predicted by response surface analysis, proving the stability and rationality of the process, and providing the theoretical basis for the process conditions of extracting anemonin from pulsatilla chinensis

Summary

Introduction

Pulsatilla chinensis (Bunge) Regel, ranunculaceae perennial herbs have the effect of clearing heat, detoxifying, cooling blood and stopping dysentery. Under the action of enzymatic hydrolysis, Ranunculin is decomposed into proto-Pulsatillarin, which is rapidly polymerized into dimer Pulsatillarin in air; at the same time, the main antimicrobial components of Pulsatilla chinensis are protoanemonin and anemonin [4,5,6], which have strong antimicrobial activity [7]. It is mainly manifested in the control of various bacteria [9,10,11] such as corn leaf spot pathogen [8], Staphylococcus aureus, etc. Chinensis methanol extract, were as follows: methanol volume fraction 87%, extraction time 2 h, solid-liquid ratio 20 (V: m), the optimum extraction amount was 3.760 μg·g-1

Objectives

Methods

Results

Conclusion