Abstract
Herein the extraction method of fucoidan from Kappaphycus alvarezii algae is reported. The fresh algae was collected from Dam Mon area (Khanh Hoa province). It was dried to 9.8% moisture and seived to 1mm in homogeneous size. Ethanol 80% was used to exclude pigments and lipids. The conditions of solvent, temperature, time for fucoidan extraction was studied. Fucoidan from Kappaphycus alvarezii was extracted by HCl, solvent rate 1/40 (w/v), 800C during 3 hours at once. The content of fucoidan extract was 40.5µg/ml. The response surface methodology (RSM) was applied to optimize 3 factors of mateial/solvent ratio, temperature and time for the extraction. The optimal results was HCl 1.39M at 85.10C in 3.64 hours, fucoidan content was 47.9 µg/ml. The FT-IR spectrum of fucoidan contain specific peaks of sulfate group in fucoidan in axial or equatorial position.
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