Study on the Judgment of Cell Detachment Using Image Processing

Abstract

This study combines image processing and enzyme to conduct the research and develope a detection system for adhesive cells detachment. First, a PCI video capture card is used to capture the microscope images into the computer, a program is edited to detect cell morphology and determine the detachment status of the cells, and then cell morphology was compared by detection data (roundness and centroid locating), in order to establish rules to detect the cell suspensions. This experiment will find the boundary sensitivity parameter, “fudgeFactor”, and the rules for the detection of cell suspension. The multiple displacement difference compliance rate (90%) is greater than displacement difference compliance rate (70%), and compared with the roundness detection, then cell detachment can be accurately measured.