Study on the Fingerprint of Stellarlae Radix by UPLC

Abstract

Objective: The UPLC fingerprint of Stellarlae Radix was established to provide reference for quality control and evaluation of Stellarlae Radix. Method: The chromatographic separation was performed on a Agilent ZORBAX Eclipse Plus C18 (2.1mm×100mm, 1.8μm) with a gradient elution program using a mixture of acetonitrile and 0.1% phosphoric acid water as mobile phase at 225 nm wavelength, the flow rate was 0.1mL/min and the column temperature was 30°C. The Injection volume 1μL. 10 batches of Stellarlae Radix were measured under the same chromatographic conditions, and the similarity evaluation of 10 batches of Stellarlae Radix was carried out by using the software traditional Chinese medicine chromatographic fingerprint similarity evaluation system (version 2004). Results: The method for UPLC fingerprint analysis of 10 batches of Stellarlae Radix was established, and the similarity was between 0.941 and 0.980, and 13 common peaks were calibrated. Conclusion: The fingerprint method established in this study has good precision, repeatability, stability, short analysis time and strong specificity, which can provide scientific basis for the quality evaluation and control of Stellar Radix.