Study on UPLC Fingerprint of Alpinia officinarum Hance

Abstract

Objective: The UPLC fingerprint of Alpinia officinarum Hance was established to provide reference for evaluating the quality of Alpinia officinarum Hance on the market. Method: Agilent ZORBAX Eclipse Plus C18 (2.1 mm ×100 mm,1.8μm) chromatographic column, mobile phase 0.01% phosphoric acid solution (A) - acetonitrile (B), flow rate 0.1ml·min-1, detection wavelength 254 nm, column temperature 30°C. The fingerprints of 10 batches of Alpinia officinarum Hance were determined, and the similarity of fingerprints was compared by using the “similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine” (2004 A). Results: The specific UPLC fingerprint of Alpinia officinarum Hance was established, 17 common peaks were established, and the similarity was between 0.922-0.989. Conclusion: The method is simple, efficient and reliable, which provides an effective and rapid evaluation method for the quality control of Alpinia officinarum Hance.