Abstract
Candida antarctica Lipase B (CALB) has a wide range of applications in many fields. In this study, Pichia pastoris was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental conditions for the harvest of CALB. The results showed that the optimal technological conditions for the foam separation and recovery of CALB were as follows: liquid volume was 150 mL, separating gas velocity was 600 mL/min, pH value was 7, and surfactant SDS concentration was 0.5 mg/mL. Under these conditions, the enrichment ratio of CALB was 0.95, and recovery rate R was 80.32%, respectively, indicating that the foam separation technology is feasible to extract lipase B.
Highlights
IntroductionIn recent years, increasing attention has been paid to the production and application of Candida antarctica Lipase B (CALB)
After centrifuging the fermentation broth cultured in the shake flask, the supernatant was taken for the foam separation experiment
After centrifuging the fermentation broth cultured in the shake flask, the supernatant was taken for foam separation experiments
Summary
In recent years, increasing attention has been paid to the production and application of CALB. CALB catalysis has a good stability and selectivity as well as mild reaction conditions, and its application covers many fields including food industry [3], household chemical industry [4], organic synthesis [5], biomedicine [6] and so on. Foam separation technology is a new separation technology developing rapidly in the recent 20 years [10]. By separating the foam layer and the liquid phase, it can purify the liquid phase and concentrate the surface-active substances (in the foam layer). The application field of foam separation technology is constantly expanding, mainly being used in wastewater treatment, food, biology, medicine, chemical industry, etc.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call