Study on the early diagnosis of HDN in ABO blood-group system

Abstract

Objective To study the early diagnosis of heamolytic disease of newborn(HDN) by umbilical blood immunoserological test. Methods According to the laboratory rules, immunoserological tests were practised with neonatal umbilical blood of 916 A or B blood-type neonates delivered by O-type blood mothers. The morbidity and the pathogenic time of hyperbilirubinemia were studied in 7 days after birth to the neonates whose erythrocyte was sensitized by the anti-A(B) IgG. Results (1) There was significant difference in the morbidity of hyperbilirubinemia of the neonates whose umbilical blood antibody-released test results were postive compared with control group (P<0.01), There had no significant difference in the morbidity of hyperbilirubinemia of the neonates compared with puerperal times. (2) There had significant difference in the morbidity of hyperbilirubinemia between the neonates having free antibody and those without free antibody (P < 0.01). (3) The peek time of hyperbilirubinemia was in 1-3 days after birth in patient-infants whose antibodyreleased test result was positive. Conclusion The umbilical blood immunoserological test is an exact method of the early diagnosis to HDN. The free antibody helps to estimate the developing trend of HDN. Key words: Antibody-released test; HDN; Hyperbilirubinemia