Correlation of the expression of HSP70 in peripheral blood and umbilical cord blood with the premature and parturient pregnant women with histologic chorioamnionitis and study on its clinical significance

Abstract

Objective To investigate the correlation of the expression of heat-shock protein 70 (HSP70) in peripheral blood and umbilical cord blood with the premature and parturient pregnant women complicated by histologic chorioamnionitis (HCA) and to study its clinical significance. Methods Thirty-four premature and parturient pregnant women with HCA were selected as premature delivery combined with HCA group and 34 premature and parturient pregnant women were selected as preterm birth group. In addition, 34 cases of full-term maternity in the same period were selected as control group. The peripheral blood and umbilical cord blood of three groups were extracted to determine the expression level of serum HSP70. The maternal and infant outcome and the expression level of HSP70 in peripheral blood and umbilical cord blood of three groups were compared. The correlation between HCA and HSP70 expression in peripheral blood and umbilical cord blood of patients in preterm delivery was analyzed. Results The incidences of puerperal infection (5.88%) and postpartum hemorrhage (5.88%) in premature delivery combined with HCA group were higher than those in preterm birth group and control group, but the difference was not statistically significant (P>0.05). The incidence of neonatal sepsis in premature delivery combined with HCA group (14.71%) was higher than that in preterm birth group and control group, the differences were statistically significant (all P<0.05). There were significant differences in the expression of HSP70 in peripheral blood and umbilical cord blood among the three groups (all P<0.05). Multiple comparison showed that the expression level of HSP70 in peripheral blood and umbilical blood of preterm birth group was higher than that of premature delivery combined with HCA group, the differences were statistically significant (t1=17.018, t2=13.939, P<0.05); the expression level of HSP70 in peripheral blood and umbilical cord blood of premature delivery combined with HCA group was higher than that of preterm birth group, and the differences were statistically significant (t1=13.397, t2=14.636, P<0.05). Pearson test showed that there was a significant positive correlation between HCA and expression of HSP70 in peripheral blood and umbilical cord blood of the patients in preterm delivery (P<0.05). Conclusions The expression of HSP70 in peripheral blood and umbilical cord blood in the premature and parturient pregnant women complicated by HCA increases abnormally. It may be associated with preterm and neonatal sepsis. The clinical intervention can be made by dynamically measuring the expression level of HSP70 in peripheral blood and umbilical cord blood, so as to reduce the risk of adverse outcomes such as neonatal sepsis, and ensure the health of mothers and infants. Key words: Heat-shock protein 70; Premature and parturient pregnant women; Histologic chorioamnionitis; Correlation