Abstract
ObjectiveTo investigate the correlation between hTREC and human papillomavirus (HPV) load and cervical intraepithelial neoplasia (CIN) grade II/III lesions and cervical cancer.MethodsA total of 135 patients with cervical lesions of different degrees admitted to our hospital from January 2016 to February 2017 were selected, including CIN I/III 65 cases, grade III 39 cases, and cervical cancer 31 cases. The expression of hTERC gene was detected by fluorescence in situ hybridization (FISH) in three groups, and the HPV load was detected by second‐generation hybridization capture (HC II) method, and its relationship with cervical lesion grade was analyzed. Department.ResultsThe positive expression rate of hTERC gene amplification was cervical cancer > CIN I/II lesion > CIN III lesion; the positive expression rate of HPV was cervical cancer > CIN I/II lesion > CIN III lesion. After treatment, the positive rate of hTERC gene amplification and HPV expression decreased significantly within 1 year (P < .05). Spearman's analysis showed that the degree of cervical lesion was positively correlated with hTREC and HPV load (P < .05).ConclusionhTREC and HPV are closely related to the occurrence and development of cervical precancerous lesions and cervical cancer. The abnormal amplification of hTERC gene increases with the grade of cervical lesions. Both of them can be used as auxiliary indicators for early screening, treatment, and prognosis of cervical cancer.
Highlights
Cervical cancer is one of the most common malignant tumors in gynecology, second only to breast cancer, and its morbidity and mortality rate ranked second, and showed a gradually increasing and younger trend.[1]
HTERC gene detection was performed by fluorescence in situ hybridization (FISH) in patients with different cervical cervical intraepithelial neoplasia (CIN) lesions, and the hybrid capture two generation test (HC2) was used to detect their human papilloma virus (HPV) load, which were analyzed in order to provide evidence for the diagnosis and treatment of cervical cancer and precancerous lesions
From the positive rate of amplification, it can be concluded that the positive rate of CIN I/II lesion group CIN III lesion group Cervical cancer group χ2 P
Summary
Cervical cancer is one of the most common malignant tumors in gynecology, second only to breast cancer, and its morbidity and mortality rate ranked second, and showed a gradually increasing and younger trend.[1]. Diagnosis and effective prognosis of cervical cancer are of great significance for patients' long-term survival, especially for the early detection, early treatment of cervical intraepithelial neoplasia (CIN), as well as the improvement of its screening accuracy, are important for the prevention and treatment of cervical cancer.[2,3]. HTERC is the main part of telomerase, and its expansion will cause cervical cells to be very prone to atypical development, and evolve into cervical cancer.[5]. HTERC gene detection was performed by fluorescence in situ hybridization (FISH) in patients with different cervical CIN lesions, and the hybrid capture two generation test (HC2) was used to detect their HPV load, which were analyzed in order to provide evidence for the diagnosis and treatment of cervical cancer and precancerous lesions
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