Study on the consistency of phenotype and genotype of Mycobacterium tuberculosis in the prognosis of patients

Abstract

Objective Regarding the bacterial strains of 49 patients with pulmonary tuberculosis, phenotypic and genotypic detections of drug sensitivity were done for rifampicin, isoniazide, ethambutol, and streptomycin.To explore whether its consistency was related to the prognosis of disease and to provide evidence for clinical treatment. Methods For the 49 patients with pulmonary tuberculosis who were received by the Tuberculosis Department from May 2009 to October 2015, their 98 clinically isolated mycobacteria tuberculosis were collected.Next, drug sensitive test was done for mycobacteria tuberculosis and polymerase chain reaction (PCR) reverse dot-blot hybridization was adopted to quickly detect the drug resistance genes of mycobacteria tuberculosis.Based on the clinical prognosis, the patients were divided into the effective group and the ineffective group, with consistency examination on the two detection methods.Next, to explore whether its consistency was related to the prognosis of disease. Results The phenotypic detections and the genotypic detections of rifampicin showed poor consistency in the test.Regarding the effective group, its consistency for the detections of drugs was higher than that of the ineffective group. Conclusions The stability of mutation sites of mycobacteria tuberculosis is connected with the prognosis of diseases. Key words: Mycobacteria; Tuberculosis; Drug susceptibility test; Phenotype; Genotype