Abstract
Brown rust disease is one the most important fungal disease of wheat in Iran. Using of resistance cultivars is the best control method of disease. In this study, genetic resistance of 82 promising lines was evaluated in green house condition. Their resistance components included Infection Type, Latent Period, Postule Size and Postule Density in the randomised complete block design with two replications. In the molecular level for lines with corresponding adult plant resistance Lr34 gene, PCR process by means of STS marker and the primer Lr34/Yr18 was done. The results of variance analysis obtained implicated a significant difference in the 1% probable. The minimum value detected as −0.88 to the Gorgan isolate and the maximum value for −0.94 to Ahwaz isolate. In the cluster analysis related to two isolates, lines were divided to four groups composed. The presence of gene Lr34 was proved into the two genotypes “ER-S8–90” and “ER-N3–90”.
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