Study on single nucleotide polymorphism polymorphism of key enzymes in folic acid metabolism in Han and Buyi women in Guiyang City

Abstract

Objective To study the characteristics of the key enzyme folic acid metabolism MTHFR (C677T, A1298C) and MTRR (A66G) in the women of the Buyi and Han ethnic groups in Guiyang City, Guizhou Province, and to provide reference for guiding the local folic acid health during pregnancy. Methods Totally 4143 fertile women including 201 Buyi women and 3942 Han women were selected from February 2017 to June 2018 in the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine. Genomic DNAs were obtained from oral mucosa cells. Detections of MTHFR and MTRR gene polymorphisms were conducted with Taqman-MGB technology. Genotype and allele frequency were compared with other ethnic minorities women that have been reported before. Results The genotype frequencies and allele frequencies of MTHFR (C677T, A128C) sites in Han women of Guiyang City were significantly different from those of Han women and local Buyi women in Zhengzhou, Urumqi, Zhuhai, Changchun and Langfang (P<0.05). Among them, the high-risk genotype TT (14.05%) of MTHFR C677T in Han women in Guiyang was significantly lower than that in Zhengzhou (40.7%), and was higher than that in Zhuhai Han women (5.71%) and local Buyi women (8.46%); MTHFR 677TT type in Guiyang Buyi women (8.46%) was significantly lower than that in Yanbian Korean (18.77%), Yinchuan Hui (16.53%), Sinan Miao (12.93%), Dali Bai (14.54%), Liaoyuan Man (26.00%), and was higher than that in Sanya Li (3.38%), there were statistical differences (P<0.05). MTRR 66GG type in Guiyang Buyi women (6.97%) was lower than that in Xinjiang Uygur (16.48%) and Sanya Yi (13.04%) with significant differences (P<0.05). Conclusion MTHFR (C677T, A1298C) and MTRR (A66G) genotype frequency in Guiyang Han and Guiyang Buyi women were different from others minorities, which could guide for a more individualized folic acid supplement program for different ethnic women. Key words: Guiyang; Han; Buyi; 5,10-Methylenetetrahydrofolate reductase; Methionine synthase reductase