SNP polymorphism of key enzymes in folic acid metabolism in Yi and Han women in Liupanshui City Guizhou Province

Abstract

Objective To study the metabolism level of folic acid between Yi and Han women in Liupanshui, and to guide supplement of folic acid. Methods Totally 1 054 fertile women were selected including 104 Yi women and 950 Han women. Genomic DNA was obtained from the oral mucosa cells. Genetype and allele frequency of5,10-methylenetetrahydrofolate reductase(MTHFR) (C677T, A1298C) andmethionine synthase reductase(MTRR) (A66G) were detected by Taqman-MGB technology. Genetype and allele frequency were compared with other ethnic minorities women in China Southwest which have been reported before. Results Genetype and allele frequency of MTHFR C677T in Liupanshui Yi women were different from Liupanshui Han and other minorities (Sinan Miao women, Sinan Tujia women, Dali Bai women). MTHFR 677CT/677TT genetype frequency in Liupanshui Yi women were lower than that in Han and others minorities. Conclusion MTHFR 677CT/677TT genetype frequency in Liupanshui Yi women were lower than that in Han and others minorities, which could guide for a more individualized folic acid supplement program for different ethnic women. Key words: Liupanshui Yi women; Liupanshui Han women; 5,10-Methylenetetrahydrofolate reductase (MTHFR); Methionine synthase reductase (MTRR)