Abstract
Ageing is a natural and inevitable process accompanied by different diseases. Medicinal lungwort (Pulmonaria officinalis) is a source of bioactive substances that can slow down ageing and improve the quality of human life. Callus culture of this plant can be a promising raw material to produce nutraceuticals. However, a composition of individual bioactive substances in medicinal lungwort has been studied insufficiently, particularly when it comes to phenolic compounds with potential geroprotective properties. We performed a study on the phytochemical composition of the callus cultures, extracted from the medicinal lungwort plant, for the presence of bioactive substances with the potential geroprotective properties and determined their quality profile. Twofold extraction of Pulmonaria officinalis callus cultures was carried out on a water bath with 70% ethyl alcohol. Determination of the safety profile of extracts was performed according to the requirements of the Pharmaceutical Norms and Regulations. Organoleptic, physicochemical and microbiological properties were considered important indicators of the quality of the extracts. It was demonstrated that the content of heavy metals, radionuclides, dry residue, residual alcohol content, as well as organoleptic characteristics and microbiological purity, comply with the regulatory documents. The phytochemical composition of the callus culture extract was determined by high-performance liquid chromatography and threelayer chromatography. The following flavonoids (rutin, isorhamnetin, quercetin), triterpenoid saponins and phenolic acids (p-coumaric, ferulaic, gallic, caffeic, rosmarinic and chlorogenic) were identified. The presence of triterpenoid saponin, p- coumaric, ferulaic and gallic acids is discovered in the extract of the lungwort callus culture for the first time. Quantitative analysis of bioactive substances showed that the content of caffeic, rosmarinic and chlorogenic acids in the callus culture extract is higher than that in the extracts from the aerial parts of the plant.
Highlights
inevitable process accompanied by different diseases
it comes to phenolic compounds with potential geroprotective properties
We performed a study on the phytochemical composition of the callus cultures
Summary
Используемое для производства нутрицевтиков сырье на основе лекарственных растений может быть загрязнено токсикантами, что является фактором риска для здоровья человека. Определение наличия в сырье тяжелых металлов – важный этап в разработке БАД на основе экстрактов лекарственных растений. Исследованные показатели безопасности экстрактов культуры медуницы по содержанию примесей тяжелых металлов: свинца (Pb), ртути (Hg), висмута (Bi), сурьмы (Sb), олова (Sn), кадмия (Cd), серебра (Ag), меди (Cu), молибдена (Mo), ванадия (V), рутения (Ru), платины (Pt), палладия (Pd), представлены в табл. 2, можно сделать вывод о том, что содержание тяжелых металлов во всех образцах экстрактов каллусных культур медуницы лекарственной не превышает предельно допустимого уровня. Экстракты соответствуют установленным требованиям ОФС.1.4.1.0021.15 «Экстракты», ОФС 1.5.3.0009.15 «Определение содержания тяжелых металлов и мышьяка в лекарственном растительном сырье и лекарственных растительных препаратах». Результаты определения микробиологической чистоты экстрактов каллусных культур представлены в табл. Содержание примесей тяжелых металлов в экстрактах каллусных культур медуницы лекарственной (Pulmonaria officinalis)
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