Study on resurrection callus culture extract on the expression and mechanism of AQP-1 in Schwann cells after injury of the resurrection

Abstract

Objective:To investigate the mechanism of AQP1 in Schwann cells and repair effect of resurrection callus culture extract on hypoxic injury cells. Method:Being cultured for 24 h, the morphological changes of the cells with different concentrations of CoCl2 were observed under the microscope and the changes of AQP1 were detected by Western blotting. Then being cultured for 24 h, the cells with different concentrations of esurrection callus culture extract were detected by CCK-8. By selecting the best concentration of resurrection callus culture extract and CoCl2, the experiment has to be continued. The changes of AQP1 and HIF-1 alpha protein were detected by Western blotting in 7 days. Result:6 μl/ml CoCl2 was selected as the optimal concentration of hypoxia model, not only because of the obvious changes in cell morphology, but no significant inhibition of cell growth, and the highest expression of AQP1. The optimum concentration of 2.5 μl/ml resurrection callus culture extract could significantly promote cell proliferation. Schwann cells increased expression of AQP1 induced expression of HIF-1 alpha, and resurrection callus culture extract can reduce AQP1 and HIF-1 alpha. Conclusion:There was a significant correlation between the expression of AQP1 and peripheral nerve Schwann cell edema, the expression of AQP1 was induced by HIF-1 after cells hypoxia. The resurrection callus culture extract can significantly decrease the expression of AQP1 and to alleviate edema in Schwann cells.