Expression of AQP-1,AQP-3, AQP-4 and AQP-5 in pulmonary tissues of mice with endotoxin-induced acute lung injury

Abstract

Objective:To study the expression of AQP-1,AQP-3,AQP-4 and AQP-5 in the pulmonary tissues of mice with acute lung injury(ALI)induced by lipopolysaccharide(LPS)and to clarify the relationship of ALI with the expression of AQP-1,AQP-3,AQP-4 and AQP-5.Methods:Forty healthy male C57BL/6 mice were evenly randomized into 5 groups:LPS 4 h group,LPS 6 h group,LPS 8 h group,LPS 10 h group and control group.ALI model was induced with LPS in all the LPS groups.Real-time PCR was used to observe the expression changes of AQP-1,AQP-3,AQP-4 and AQP-5 mRNA.Immunohistochemical method and Western blotting assay were used to determine the changes of AQP-1,AQP-3,AQP-4 and AQP-5 protein in the pulmonary tissues of all the animals.Meanwhile,measurement of lung wet/dry(W/D)weight ratio and pathological staining were performed in each group.Results:The W/D values of the LPS 4 h,6 h,8 h and 10 h groups(4.39±0.19,4.58±0.17,4.87±0.21 and 5.28±0.16,respectively)were significantly higher than that of the control group(3.99±0.25,all P0.05).The expression of AQP-1 and AQP-5 protein in the lung of the LPS 4 h group decreased to(74.1±5.2)% and(70.3±7.1)% that of the control group;the expression of AQP-1 and AQP-5 protein in the lung of the LPS 8 h group decreased to(45.2±4.4)% and(38.6±8.9)% that of the control group,and the expression of AQP-3 and AQP-4 had no obvious changes.Conclusion:AQP-1 and AQP-5 may play important roles in the abnormal ALI fluid transportation and might be associated with the development of pulmonary edema.AQP-3 and AQP-4 may not participate in the development of pulmonary edema during ALI.