Study on pure red cell aplastic lymphocyte subsets characteristics and the effect of cyclosporin A

Abstract

Objective To detect and analyse acquired pure red cell aplasia (PRCA) T lymphocyte subsets distribution and to assess its condition and cyclosporine immune function of T lymphocytes subsets. Methods Flow cytometry was applicated to detect peripheral blood T lymphocyte subsets of acquired PRCA patients before and after 3 months of treatment with cyclosporine-based immunosuppressive regimen and normal controls. Results Among 22 patients, 17 cases of blood returning normal (three cases of bone marrow returning normal), the total effective rate was 95.5 % (3 cases of cure, 14 cases of remission, 4 cases of improvement, 1 case of ineffectiveness). Th (CD3+ CD4+) cells and Th/Ts ratios in acquired PRCA patient group were lower than those in the normal control group, while Ts (CD3+ CD8+) cells was higher than that in the normal control group, the differences were statistically significant (P< 0.05). After 3 months of treatment, Th cells and Th/Ts ratio were higher than those before, and Ts cells were decreased compared with the previous (P< 0.05). Conclusion Disorders of T lymphocyte subsets in acquired PRCA patients lead to immune dysfunction, however, cyclosporine can improve T lymphocyte subsets in patients with imbalance, which is an effective way to treat the disease with significant curative effect and mild adverse reactions. Key words: Acquired pure red cell aplastic; T lymphocytes; Cyclosporine