Study on p73 gene polymorphism and genetic susceptibility to hepatocellular cancer

Abstract

Objective To evaluate the potential role of the separately p73 gene polymorphism on hepatocellular carcinoma(HCC) susceptibility. Methods A hospital-based case-control study was conducted in 100 cases HCC patients and 100 cases age, sex-matched cancer-free controls in the same region.The matrix-assisted laser desorption ionization time-of-flight mass spectrometry and DNA sequencing methods were used to analyze polymorphisms of p73 genetic polymorphisms (G4C14-A4T14). Using χ2 test to exam the differences of genotype between the HCC group and control group, logistic regression was used to analysis odds ratio(Oddsratios, OR) and 95%CI(Confidence Intervals, CI), adjusted for age and sex, analysis the association between SNPs of P73 polymorphisms and HCC.All the results were analyzed by SPSS 18.0. Results (1)The differences of age(χ2=0.185, P=0.667), gender(χ2=0.026, P=0.873), drinking status(χ2=0.427, P=0.514), and family history (χ2=0.058, P=0.809)of cancer composition between HCC group and control group were not statistically significant.(2)There were CC, CT and TT three genotypes in p73 rs1801173 C/T.The frequencies of CC, CT and TT genotypes of P73 C/T in controls and HCC cases were 65.0%, 28.2%, 6.8 % and 62.2%, 31.1%, 6.7%, respectively, and there was no significant difference between the two groups(χ2=0.326, P=0.568). (3)When stratifying by age, gender, smoking status, alcohol consumption, HBV carrier status and family history of cancer we found that the variant genotypes of GC/AT + AT/AT in P73 was associated with a significantly increased risk of HCC among HbsAg-positive individuals(χ2=3.916, P=0.048) and female(χ2=6.545, P=0.01), and there were no significant correlation with age, smoking, alcohol consumption, family history of cancer. Conclusion (1)p73 G4C14-to-A4T14 dinucleotide polymorphism may play a role in the development of chronic HBV-infected HCC in the Chinese population.(2)p73 G4C14-to-A4T14 dinucleotide polymorphism may play a role in the development of chronic HCC in female of Chinese population. Key words: Gene polymorphism; p73; Hepatocellular carcinoma