Study on optimization of extraction process and resistance to oxidation of Polypeptide from sea cucumber waste liquid

Abstract

The protease hydrolysis technology is used to extract the polypeptides from the sea cucumber waste liquid. The enzymatic hydrolysis ability of protein in sea cucumber waste liquid by seven proteases shows that the best protease is compound protease by taking the hydrolysis degree as the index. Furthermore, the effects of enzyme dosage, pH, temperature and time of enzymatic hydrolysis on the hydrolysis degree are studied, and Box-Benhnken central composite test and response surface analysis method are used to optimize the enzymatic hydrolysis conditions.. The results show that the optimal enzymatic hydrolysis conditions are: enzyme dosage: 647 U/g; pH: 6.48; temperature: 52.5°C; enzymatic hydrolysis time: 3.44h; protein hydrolysis degree under the best condition: 25.9%. The polypeptides extracted from the sea cucumber waste liquid have high antioxidant activity, and the DPPH· scavenging activity increases with the polypeptide concentration.