Optimization of Enzymatic Hydrolysis of Freeze-Dried Sea Cucumber Powder with Response Surface Methodology and Its Antioxidant Evaluation

Yahui Pang,Qian Liu,Jianfeng Sun

doi:10.3844/ajbbsp.2016.241.252

Abstract

In this study, freeze-dried sea cucumber powder was used as raw material and the preparation processing conditions were studied for hydrolysis of sea cucumber powder with neutral protease using Response Surface Methodology (RSM) to obtain a high concentration of sea cucumber polypeptide. The effects of hydrolysis temperature, pH, enzyme dosage and hydrolysis time on the degree of hydrolysis (DH) were studied. Based on the single factor experiment, Box-Benhnken center composite experiment was carried out with three factors and three levels, including hydrolysis temperature, pH and enzyme dosage. RSM was used to determine the effect of prime factors on the DH and establish a regression model. The results showed that the optimal hydrolysis conditions were as follows: enzyme dosage of 5753 U g-1 dry matter, hydrolysis temperature of 52Â°C, pH of 8.3, substrate concentration of 5% and hydrolysis time of 5 h. Under the optimal conditions, the DH was up to 16.01%, which matched well with the predicted value. With VC as the positive control, the scavenging ability of the hydrolyzate on DPPH radicals, hydroxyl radicals and superoxide radicals were very powerful, which mean that the hydrolyzate of sea cucumber powder has good antioxidant activity.

Highlights

As the traditional healthy seafood in East Asian countries, sea cucumber is an important marine resource of food and drugs (Farouk et al, 2007; Bai et al, 2013)
The degree of hydrolysis (DH) of vacuum freeze-dried sea cucumber powder was performed with a threevariable, three-level Box-Behnken design based on the Response Surface Methodology (RSM)
The statistical analysis showed that the optimal hydrolysis conditions were as follows: Enzyme dosage of 5753 U g−1 dry matter, hydrolysis temperature of 52°C, pH of 8.3, substrate concentration of 5% and hydrolysis time of 5 h

Summary

Introduction

As the traditional healthy seafood in East Asian countries, sea cucumber is an important marine resource of food and drugs (Farouk et al, 2007; Bai et al, 2013). The major edible and medicinal parts of sea cucumber are its body walls mainly consisting of polypeptide, mucopolysaccharide, sterols, saponins, fatty acids and other biologically active substances (Cui et al, 2007; Yu et al, 2015). Those active substances have physiological activity such as anti-tumor, reducing blood pressure, anti-oxidation, improving immunity, reducing joint pain and anti-aging (Zhao et al, 2007). As the typical high-protein, low-fat, lowcholesterol and rich in minerals and vitamins highquality seafood, sea cucumber is an ideal raw material for the preparation of bioactive peptides. Neutral protease opens the peptide bond of protein mainly internally, which can position hydrolytic cleavage to produce a specific peptide in mild conditions, with the characteristics of moderate hydrolysis potency, high safety, does not destroy the

Methods

Results

Conclusion