Abstract
The paper uses a method that combines biological characteristics and 16S rDNA sequence characteristics to analyze potato scab bacteria collected from China. The study found that the obtained universal detection primers B1/B2 showed good specificity for all pathogenic strains, and could stably expand the target band, while non-pathogenic strains had no bands. The paper uses the spore dilution method to verify that the established detection method has a sensitivity of 20 pg·μL-1 for strain DNA, and the detection threshold for spores is about 4.0 CFU/μL. The genome amplification results of the test samples showed that the target bands were detected in the scab pathogen Streptomyces, diseased potato sample tissues, and diseased soil samples, but not the scab pathogen Streptomyces, healthy tuber tissues, non-sick soil samples and other strains None of the bands of interest were expanded, indicating that the method has good specificity. Therefore, we can conclude that the qualitative detection method of potato scab bacteria can realize rapid detection of strains, diseased tissues and soil samples.
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