Abstract
A nutritional feeding experiment with synthetic astaxanthin and Haematococcus pluvialis supplemented was conducted to determine growth performance, antioxidant capacity and anti-inflammation in golden pompano (Trachinotus ovatus).Three diets were formulated with or without H. pluvialis and synthetic astaxanthin supplemented to feed fish for 8 weeks. As the results showed, growth performance (FBW, WG and SGR) significantly increased with synthetic astaxanthin and H. Pluvialis supplemented. Feed coefficient ratio (FCR) of T. ovatus fed diets with H. Pluvialis and astaxanthin supplemented was lower than that of fish fed the control diet. The hepatic total antioxidant capacity (T-AOC) in T. ovatus fed diet with H. Pluvialis and astaxanthin supplemented was higher than that of fish fed the control one. Superoxide dismutase (SOD) and hepatic malondialdehyde (MDA) declined slightly with astaxanthin and H. Pluvialis supplementated than that in control group without significant differences. Under normal rearing experiments, there was no significant difference among the treatments in Nrf2-ARE related genes. However, after the acute hypoxia trial, the relative expression of Nrf2, Keap1, CAT, SOD, GSH-PX and HO-1 were all significantly higher in astaxanthin and H. Pluvialis treatments than that in the control group. After LPS administration, the mRNA relative expression of Nrf2-ARE related genes including Keap1, Nrf2, CAT, GSH-PX, HO-1 and SOD were all significantly higher in astaxanthin and H. Pluvialis treatments than that in the control group. The mRNA relative expression of NF-κB related genes IKK, IκB, and P65 as well as IL1β were all significantly lower in astaxanthin and H. Pluvialis treatments than that in the control group. These results indicated that dietary astaxanthin and H. Pluvialis improved the growth performance and antioxidant capacity under acute hypoxia stress, and alleviated the inflammatory response by activating Nrf2-ARE pathway to antagonize the NF-κB pathway.
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