Abstract

BackgroundTraditional measurements of apple seedling roots often rely on manual measurements and existing root scanners on the market. Manual measurement requires a lot of labor and time, and subjective reasons may cause the uncertainty of data; root scanners have limited scanning size and expensive. In case of fruit roots, coverage and occlusion issues will occur, resulting in inaccurate results, but our research solved this problem.ResultsThe background plate was selected according to the color of the seedling roots; the image of the roots of the collected apple seedlings was preprocessed with Vision Development Module by combining image and Labview. The root surface area, average root diameter, root length and root volume of apple seedlings were measured by combining root characteristic parameters algorithm. In order to verify the effectiveness of the proposed method, a set of measurement system for root morphology of apple seedlings was designed, and the measurement result was compared with the Canadian root system WinRHIZO 2016 (Canada). With application of SPSS v22.0 analysis, the significance P > 0.01 indicated that the difference was not significant. The relative error of surface area was less than 0.5%. The relative error of the average diameter and length of the root system was less than 0.1%, and the relative error of the root volume was less than 0.2%.ConclusionsIt not only proved that the root surface area, average root diameter, root length and root volume of apple seedlings could be accurately measured by the method described herein, which was handy in operation, but also reduced the cost by 80–90% compared with the conventional scanner.

Highlights

  • ResultsThe background plate was selected according to the color of the seedling roots; the image of the roots of the collected apple seedlings was preprocessed with Vision Development Module by combining image and Labview

  • Apple is one of the four major fruits in the world

  • Traditional measurements of apple seedling roots often rely on manual measurements and existing root scanners on the market

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Summary

Results

T Test The "Independent Sample T-Test" process test is primarily used to test whether two samples are from a subject with the same average. From the variance homogeneity levin test results of root surface area, average root diameter, root length and root volume, it could be seen that the data variance obtained from the two measurement methods was equal; assuming the T test results with equal variance, i.e. All the P values were greater than the significance level of 0.01, it could be judged that there was no. Error analysis In this study, WinRHIZO 2016 (Canada) was used as the test standard to calculate the standard deviation, absolute error and relative error of root surface area, average diameter, length and volume measured by the software. This study developed and tested an image processing system to measure the morphological parameters of apple seedling roots (surface area, average diameter, length, and volume) accurately. Compared with WinRHIZO 2016 measurement results, measurement results of root surface area, average root diameter, root length and root volume R2 in this analysis system were 0.994, 0.993, 0.995 and 0.993, indicating that the. This analysis software system designed could be used to measure the morphological parameters of apple root system

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