Abstract
Citrus grandis cv. ‘Hongmianmiyou’ is mutated from ‘Guanximiyou’, which has been widely cultivated in Sichuan Province because of the attractive appearance, rich nutritional ingredients and trace elements. To effectively cultivate more Hongmianmiyou seedlingsfor production, we established the callus tissue induction system. The basic nutrient medium consisted of MS medium with 3% of sucrose and 0.8% of agar, adjusted to pH 5.8.Various concentrations of 6-benzylaminopurine and 2,4-dichlorophenoxyacetic, as well as polyvinyl pyrrolidone and active carbon, and culture condition were carried out to developprotocols for the in vitro propagation of pummelo. The appropriate formula was MS mediumsupplemented with 1 mg•L−1of 2,4-D,0.5 mg•L−1of 6-BAand 0.05% of active carbon. Explants were cultured maintained under a 16 h photoperiod provided by cool white fluorescent light 1200 Lux after cultured at dark for 7 days. Light green compact callus induced from semi-lignified stems explant of spring shoots in ‘Hongmianmiyou’, reaching 97% induction rate.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
