Anti-browning treatments and induction on explants of Dendrobenthamia hongkongensis

Abstract

To study the effects of different types of explants, sterilization time, basic mediums, pretreatment methods, and anti-browning agent types and their concentration on browning of three explants, Dendrobenthamia hongkongensis with young leaves, with stem segments, and with stem segments having buds, were used as treatments. This research also studied the effect of 6-Benzylaminopurine (6-BA), 1-Naphthaleneacetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) on induction of the three explants through tissue culture technology. Results showed that disinfecting explants for five min with 1.0 g·L-1 Mercury dichloride solution was the best way of disinfection with woody plant medium (WPM) being the most suitable basic culture medium. When soaking explants in 1.0 g·L-1 polyvinyl pyrrolidone (PVP) for 3.0 h, the browning rates of the three explants were greatly decreased(P < 0.05). For young leaves, 1.0 g·L-1 citric acid (CA) was the best anti-browning agent having a browning rate of 27.4%; 2.0 g·L-1 PVP was the best anti-browning agent for stem segments (browning rate of 13.3%) and stem segments with buds (browning rate of 16.7%). The best callus induction medium for young leaves was WPM + 1.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1 2, 4-D, and the best induction medium for stem segments and stem segments with buds was WPM + 2.0 mg·L-1 6-BA + 0.5 mg·L-1 2, 4-D.