Study on High-Risk Human Papillomavirus Infection in Naxi Women of Lijiang, Yunnan Province

Abstract

Objective To investigate the status and risk of high-risk human papillomavirus (HR-HPV)infection in Naxi women of Lijiang, Yunnan Province.To determine association between HR-HPV and the stage of squamous cervical cancer (SCC) and cervical intraepithelial neoplasia (CIN) lesion.To provide the basis for local cervical cancer control. Methods From April 2009 to February 2012, a total of 6528 women who were resident in Lijiang City and had sexual experience, Outpatient gynecological census were recruited. Their HR-HPV DNA load was detected by hybrid capture 2 (HC2). HR-HVP infections of different cervical lesion stages were compared. HR-HPV DNA load was measured by the ratios of relative light units compared to cut off (RLU/CO). According to RLU/CO values, they were categorized into: Negative group ( 100.00). Association between HR-HPV and CINs were evaluated by unconditional multinomial logistic regression. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Lijiang City People' Hospital. Informed consent was obtained from all participants. Results Positive for HR-HPV was 12.55%(819/6528) among 6528 tested women. There had no significance difference between infection rates of HR-HPV and ages among 6528 women(χ2=109.21, P 0.05). HR-HPV infection rates had no significance difference between the other nationalities and local Naxi women(P>0.05). The percentage of high HR-HPV DNA load increased with the severity of cervical lesions[1.00%(5/500)in chronic inflammation of the cervix group, 3.62%(22/607)in CINⅠ group, 9.89%(43/435)in CINⅡgroup, 49.11%(55/112)in CINⅢ group, 65.38%(17/26)in SCC group], that is, cervical lesions had a positive correlation with the percentage of high HR-HPV DNA load(r=0.425, P<0.05). Conclusions Young age, first sexual behavior before 18 years old, many times of gravidities, parities and sexual partners more than two were high-risk factors of HR-HPV infection. Cervical lesions were highly influenced by HR-HPV infection and HR-HPV DNA load. Key words: high-risk human papillomavirus; hybrid capture; viral load; cervical intraepithelial neoplasia