Study on expression of RYR2, FKBP12.6 and CaMK-II in lung tissue of patients with chronic obstructive pulmonary disease

Abstract

Objective To explore the significance of immunohistochemical expression of Ryanodine receptor 2 (RYR2), FK506-binding protein 12.6 (FKBP12.6) and Calcium/ calmodulin dependent protein kinaseⅡ (CaMK-Ⅱ) in lung tissues from patients with chronic obstructive pulmonary disease (COPD). Methods A retrospective analysis from November 2012 to April 2017 in People′s Hospital of Ningxia Hui Autonomous Regin, lung tissue of 30 patients after lung cancer surgery, according to the results of pulmonary function test and Cardio-Uhrasonography, the patients were divided into three groups: COPD with pulmonary hypertension (PH)(group A) and COPD group (group B), non-COPD, non-PH patients with pulmonary tumor as the negative control group (group C). Take lung tissue about 5 cm from the cancerous area was removed after surgery.The Circum-ferential area of pulmonary valve of the three groups has been observed.To observe RYR2, FKBP12.6 and CaMK-Ⅱby immunohistochemical method respectively, then make correlation analysis. Results (1)Compared with group C, wall area% (WA%), pulmonary artery systolic pressure (PASP) were higher in group A and group B (F=24.115, 9.421, both P<0.05), and WA%, PASP were higher in group A than group B. (2)Compared with group C, the immunohistochemical expressions of RYR2, FKBP12.6 and CaMK-Ⅱ in group A and group B were all increased (F=9.219, 6.143, 11.337, all P<0.05), and the immunohistochemical expressions of RYR2, FKBP12.6 and CaMK-Ⅱ in group A was higher than group B. (3)Correlation analysis indicated that the immunohistochemical expressions of RYR2 in group A and B was positively correlated with WA% (r=0.547, 0.771, both P<0.01), while the immunohistochemical expressions of RYR2 in group A was positively correlated with PASP (r=0.773, P<0.01). Histochemical expressions of FKBP12.6 in group A were positively correlated with WA% (r=0.796, 0.810, both P<0.01), and the immunohistochemical expressions of FKBP12.6 in group A was positively correlated with PASP (r=0.729, P<0.01). The immunohistochemical expressions of CaMK-Ⅱ in group A and B were positively correlated with WA% (r=0.879, 0.504, both P<0.01), and the immunohistochemical expressions of CaMK-Ⅱ in group A was positively correlated with PASP (r=0.748, P<0.01). Conclusions FKBP12.6 and CaMK-Ⅱ may play a role in COPD-related pulmonary artery proliferation. Key words: Pulmonary disease, chronic obstructive; Hypertension, pulmonary; Ryanodine receptor calcium release channel; Calcium-calmodulin-dependent protein kinase type 2; FK506-binding protein 12.6