STUDY ON CHEMICAL QUALITY AND INHIBITORY EFFICACY OF TRIGONA BEE (Tetragonula clypearis) HONEY FROM BREEDERS IN EAST LOMBOK DISTRICT AGAINST Pseudomonas aeruginosa IN VITRO

Abstract

Chemical properties and antimicrobial activity of honey can be affected by geographic location. The East Lombok region has varied geographical conditions including mountains and lowlands. The aim of this study was to determine the chemical quality and inhibition activity of trigona honey bee from Breeders in East Lombok District against Pseudomonas aeruginosa in vitro. This study used a completely randomized design (CRD) with one factor, which consisted of 6 (six) locations, namely breeders Dames Damai, Bintang Rinjani, Sukarema, Bebidas, Banjar Sari and Geres. Data were analyzed by ANOVA at a significance level of 5% using Co-Stat software. Significantly different data were further tested with a Honestly Significant Difference (HSD). Data of phytochemical compounds (tannins and flavonoid) were analyzed using a descriptive method. The results showed that trigona honey obtained from 6 breeders in East Lombok had significantly different pH, water content and reducing sugar content. The pH of trigona honey obtained from 6 farms in East Lombok Regency is in the general pH range of trigona honey, which ranges from 2.99 to 3.33. Meanwhile, the water content of trigona honey obtained from 6 farms in East Lombok Regency was very high, namely 27.98 - 41.01% (bb) with very low reducing sugar levels, which only ranged from 25.22 - 30.92%. Therefore, all of these honey samples did not meet the quality standards based on SNI-8664-2018. The antimicrobial activity of trigona honey from 6 breeders in East Lombok showed very strong inhibition against Pseudomonas aeruginosa in vitro because of the flavonoids content.