Abstract
Gut bacterial diversity of Sitophilus oryzae, a commonly known granary insect pest was examined for their potential β-1,4-endoglucanase activity. After collection (from a granary at Tezpur Assam, India) and aseptically dissection, the insect gut content was cultured in Brain-Heart infusion agar to obtain individual bacterial colony. After morphological studies 11 isolates were subjected to PCR based molecular characterization. It was found that isolates mostly belong to Bacillus and γ-Protobacteria. Four isolates were showing the β-1,4-endoglucanase activity; with RB 5 (Bacillus subtilis strain TLO3) being the most potent at 4.5 pH and 50 °C. Again, these active isolates were studied for their growth in 35 different carbohydrates. FTIR study was carried out with RB 5 to ascertain the functional group and band pattern when treated with cellulosic biomass. The study primarily reporting on the gut bacterial diversity (culturable) and their cellulose hydrolytic potential may help towards appropriate applications for biodegradation.
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