Study on anti-biofilm activity of some commonly available plant leaf extracts on Pseudomonas species and Staphylococcus aureus

Abstract

Bacteria benefit from biofilm formation because it shields them from unfavorable circumstances. Compared to planktonic bacteria, it demonstrates a high level of antibiotic resistance. Additionally, this makes it challenging to manage the associated illnesses. Therefore, the creation of novel anti-biofilm agents is required. Compared to their chemically manufactured counterparts, antimicrobial compounds found in plants are the greatest source because they have few or no negative effects. Plant products are superior in their utility since there is less chance of development of medication resistance and there are less adverse effects. The present work is done to screen anti-biofilm activity of some commonly available plants viz. Tinospora cordifolia, Ocimum basilicum, Justicia adhatoda and Nyctanthes arbortristis on Pseudomonas sps. and Staphylococcus aureus. Phyto-chemical screening was performed for the obtained extracts. The method involved the administration of methanol extract of the plant leaves obtained by maceration procedure, on Pseudomonas species and Staphylococcus aureus. Pseudomonas species biofilm was inhibited by Tinospora and Nyctanthes arbortristis and Staphylococcus aureus biofilm was inhibited by all the four extracts. The studies revealed that all the plant leaves used here exhibit antibiofilm activity. The minimum inhibitory concentration (MIC), biofilm-inhibitory concentration (BIC) and percentage of biofilm formation inhibition were calculated, Tinospora and Nyctanthes were revealed to be the most efficient plants against both bacteria.