Abstract
The interest in determining the influence of new metal complexes on microorganisms is increasing due to the growing pathogenic resistance. This investigation showed influence of 5 new Pt(IV) complexes on 16 strains of bacteria. Antibacterial activity was tested using microdilution method with resazurin while antibiofilm activity was observed by tissue culture plate method and antibiotic doxycycline was used as positive control. The results were expressed as minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and biofilm inhibitory concentration (BIC). The complexes were labeled as: C1 for Pt(S-bz-thiosal)3, C2 for Pt(S-met-thiosal)3, C3 for Pt(S-et-thiosal)3, C4 for Pt(S-pr-thiosal)3 and C5 for Pt(S-bu-thiosal)3. The best result on Gram positive bacteria was obtained with C1 and MIC on Staphylococcus aureus ATCC 25923 was ˂7.81 μg/ml. Bifidobacterium animalis subsp. lactis (probiotic) was sensitive to C2 (MIC at 15.625 μg/ml). The best sensitivity on Gram negative bacteria was observed on Escherichia coli ATCC 25922 with C1, C2, C3 and C4, on Proteus mirabilis ATCC 12453 with C1, and on Pseudomonas aeruginosa with C2, C3 and C5 (all MICs at 250 μg/ml). The tested complexes were more efficient as antibiofilm agents and the best results were obtained with C2 acting against S. aureus and S. aureus ATCC 25923 biofilm. In conclusion, we noticed that the tested compounds exhibited promising properties as antibacterial agents and antibiofilm agents,
Highlights
The interest in determining the influence of new metal complexes on microorganisms is increasing due to the growing pathogenic resistance
Antibacterial activity was tested using microdilution method with resazurin while antibiofilm activity was observed by tissue culture plate method and antibiotic doxycycline was used as positive control
The best sensitivity on Gram negative bacteria was observed on Escherichia coli ATCC 25922 with C1, C2, C3 and C4, on Proteus mirabilis ATCC 12453 with C1, and on Pseudomonas aeruginosa with C2, C3 and C5
Summary
The interest in determining the influence of new metal complexes on microorganisms is increasing due to the growing pathogenic resistance. Investigations of other Pt complexes in microbiology have been conducted, showing wide influence on microorganisms but being more or less effective. It includes bimetallic complexes (Al-Hasani, 2007), Pt complexes as polymeric nanoparticles (Elhusseiny and Hassan, 2013), different polymers with Pt(IV) (Nartop et al, 2013), Pt(IV) chelate (Hegazy, 2012), Pt(IV) dithiocarbamate complexes (Manav et al, 2006), thiodiamines with Pt(IV) (Mishra and Kaushik, 2007), etc. The reaction mixture was heated on a water bath with stirring for 3 h During this period, small portions from a solution of LiOH (0.6 mmol with 10 ml of distilled water) were added.
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