Study of volatile fraction of Pimpinella anisum herb

Abstract

Topicality. Pimpinella anisum is widely used in folk medicine and pharmaceutical industry. The study of volatile compounds of this plant is aimed mainly at fruits and seeds, and there is insufficient data on the study of herbs.Aim. To study the qualitative composition and quantitative content of the components of the volatile fraction of the Pimpinella anisum herb.Materials and methods. For the analysis, we used Pimpinella anisum herb harvested in Kharkiv, in summer, 2019. Chromatographic separation performed on an Agilent 6890N/5973inert gas chromatography – mass spectrometric system (Agilent technologies, USA). Compounds have been identified using the NIST 02 mass spectra library. Quantitative analysis has been performed by adding an internal standard solution to the test samples.Results and discussion. The presence of 11 components has been established, the total content of which is 3.41 mg/g. The volatile fraction of the Pimpinella anisum herb is dominated by trans-anethole (1.10 mg/g), isoeugenol acetate (0.91 mg/g) and thellungianin G (0.84 mg/g). Unlike fruits and seeds of Pimpinella anisum, where the content of trans-anethole is 90-96 %, in the herbs it is almost 3 times less – 33.26 %.Conclusions. The qualitative composition and quantitative content of the components of volatile fraction of Pimpinella anisum herb have been investigated by the method of gas chromatography-mass spectrometry; 11 compounds were identified, among which trans-anethole dominates with a content of 33.26 %.