Abstract
This study was conducted to analyze the formation of DNA adduct 8-OHdG due to oxidative damage of DNA caused by Bisphenol (BPA) and chromium (VI) exposure. The in vivo study was conducted in the rat (Rattus norvegicus) group with exposure of BPA (2 mg/kg BW), the mixture of BPA (2 mg/kg BW) and Cr (VI) (1.28 µg/kg BW) for 28 days, respectively. Urine of rats was collected every week as samples for tracking the 8-OHdG compounds. The formation of 8-OHdG in urine samples was analyzed using LC-MS/MS with reverse-phase chromatography. Mixture of ammonium acetate pH 4.0 20 mM and acetonitrile were used as mobile phase in LC-MS/MS for an elution gradient. The results showed that the exposure of BPA, the mixture of BPA and Cr (VI) might cause the formation of urinary 8-OHdG in rats. The longer exposure time causes the increase level of urinary 8-OHdG formations. The study also showed that urinary 8-OHdG concentration increases due to synergistic effect between BPA and Cr (VI) exposure.
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