Abstract
A tomographic microscopy system can achieve instantaneous three-dimensional imaging, and this type of microscopy system has been widely used in the study of biological samples; however, existing chromatographic microscopes based on off-axis Fresnel zone plates have degraded image quality due to geometric aberrations such as spherical aberration, coma aberration, and image scattering. This issue hinders the further development of chromatographic microscopy systems. In this paper, we propose a method for the design of an off-axis Fresnel zone plate with the elimination of aberrations based on double exposure point holographic surface interference. The aberration coefficient model of the optical path function was used to solve the optimal recording parameters, and the principle of the aberration elimination tomography microscopic optical path was verified. The simulation and experimental verification were carried out utilizing a Seidel coefficient, average gradient, and signal-to-noise ratio. First, the aberration coefficient model of the optical path function was used to solve the optimal recording parameters. Then, the laminar mi-coroscopy optical system was constructed for the verification of the principle. Finally, the simulation calculation results and the experimental results were verified by comparing the Seidel coefficient, average gradient, and signal-to-noise ratio of the microscopic optical system before and after the aberration elimination. The results show that for the diffractive light at the orders 0 and ±1, the spherical aberration W040 decreases by 62–70%, the coma aberration W131 decreases by 96–98%, the image dispersion W222 decreases by 71–82%, and the field curvature W220 decreases by 96–96%, the average gradient increases by 2.8%, and the signal-to-noise ratio increases by 18%.
Highlights
The fast and sensitive acquisition of 3D data is one of the main challenges in modern biological microscopy
The off-axis Fresnel zone plate-based tomographic microscopic imaging system reduced the problems of sample light bleaching and light damage caused by strong incident light, and it was suitable for live-cell imaging because of its real-time image capture, which avoided the problem of error image acquisition at different times caused by excessive cell swimming
In 2011, Feng Y et al observed two swimming sperms using a tomographic microscopy system based on off-axis Fresnel zone plates, but there were chromatic aberrations and geometric aberrations in the system that affected the imaging quality of the tomographic microscopy system [5]
Summary
The fast and sensitive acquisition of 3D data is one of the main challenges in modern biological microscopy Most imaging methods, such as laser scanning confocal techniques, achieve optical laminarization by changing the planes of confocalization, which cannot be imaged at the same time because the focal planes are not recorded simultaneously [1]. In 2011, Feng Y et al observed two swimming sperms using a tomographic microscopy system based on off-axis Fresnel zone plates, but there were chromatic aberrations and geometric aberrations in the system that affected the imaging quality of the tomographic microscopy system [5]. Feng Y et al used a pre-dispersion element to correct chromatic aberration and applied it to a tomographic microscopic imaging system to solve the problem of chromatic aberration affecting imaging quality in 2013 [6].
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