Study of the in vitro release profile of sesquiterpenes from a vaginal cream containing Copaifera duckei Dwyer (Fabaceae) oleoresin

Helison Carvalho,Jose Carvalho,Anderson Sanches,Jocivania Silva,Clarissa Lima,Caio Fernandes

doi:10.7324/japs.2015.50401

Helison Carvalho, Jose Carvalho + Show 4 more

Open Access

https://doi.org/10.7324/japs.2015.50401

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Abstract

in the present study. The dissolution test revealed that trans-caryophyllene (TC) was constantly released until the final test time. The maximum peaks were found at a concentration of 478.79 nL/mL for the buffer at pH 5 and at a concentration of 475.99 nL/mL for the buffer at pH 6, with a percentage release of 50.52% and 50.21%, respectively. Likewise, the caryophyllene oxide (CO) was released into the buffers at pHs 5 and 6, with maximum release peaks at 60 minutes at a percentage of 31.8% and 33.5% corresponding to concentrations of 2.779 and 2.925 μg/mL, respectively. The release and dissolution profiles of the sesquiterpene compounds indicated that a pH of 5-6 in the dissolution media is adequate for release of these markers of antimicrobial activity in Copaifera duckei Dwyer. Therefore, these conditions possibly favor the therapeutic action of CVC.

Highlights

Copaiba trees belong to the genus Copaifera L., family Fabaceae, subfamily Caesalpinioideae
The in vitro release profile of sesquiterpenes in a vaginal cream containing copaiba (CVC – copaiba vaginal cream) oleoresin in buffered solutions at different pHs was evaluated in the present study
The release and dissolution profiles of the sesquiterpene compounds indicated that a pH of 5-6 in the dissolution media is adequate for release of these markers of antimicrobial activity in Copaifera duckei Dwyer

Summary

Introduction

Copaiba trees belong to the genus Copaifera L., family Fabaceae, subfamily Caesalpinioideae. These plants are slowgrowing trees, measuring 25-40 m in height, featuring a rough and dark trunk 0.4-4 m in diameter and living up to 400 years of age (Silvia et al, 2008). The normal physiological pH in the vagina varies between 3.8 and 4.2. These acidic values result from the secretion of organic acids by the vaginal epithelial cells and from the production of lactic acid from glycogen by bacilli and lactobacilli constituting the normal vaginal microbiota (Jarmy-Di Bella et al, 2009)

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