Abstract
Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose
Highlights
Polyhydroxyalkanoates (PHAs) as a biodegradable material are considered as alternative biopolymers for petroleum-derived plastics [Kourmentza et al, 2017]
A bacterial strain was isolated from a garden soil located in the city of Baghdad-Iraq, the ability of this isolate to produce PHA was determined using staining methods (Sudan B Black stain and Nile Blue A stain)
The results showed abundance of accumulated intracellular PHA granules in bacteria
Summary
Polyhydroxyalkanoates (PHAs) as a biodegradable material are considered as alternative biopolymers for petroleum-derived plastics [Kourmentza et al, 2017]. Synthetic plastics which have been extensively used since many decades, known as non-degradable plastics, are potent environmental toxic pollutants and have accumulated in the environment. The most abundant member of the PHAs family, known as poly-3-hydroxybutyrate (P3HB), a homopolymer of 3-hydroxybutyrarte, similar in properties to polypropylene. Poly-3-hydroxyvalerate (PHV) is another member of the PHAs family with more flexible properties [Napathorn, 2014]. PHA are thermoplastic polyesters produced by bacteria intracellularly as carbon and energy storage and capsulated as granules in cytoplasm [Radivojevic et al, 2016]. PHA was found to accumulate in large number of bacteria e.g. Ralstonia eutrophes, Pseudomonas putida, P. fluorescens, Bacillus spp. and Escherichia Coli [Poltronieri and Kumar, 2017; Pagliano et al 2017]
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