Changes in the concentration of chemical elements in the body tissues of broilers when fed with the probiotic <I>Bacillus cereus</I> and coumarin

Abstract

Relevance. Due to the cancellation of feeding animals with low doses of antibiotics, alternative substances are being searched, phytobiotics and probiotics may become promising. The use of plant-based feed additives in the nutrition of farm animals and poultry contributes to improving health, and, consequently, improving productive qualities.The purpose of the study: to study the effect of probiotic (Bacillus cereus IP 5832) and coumarin both separately and together on the concentration of chemical elements in the body tissues of broiler chickens.Methods. The object of the study was 7-day-old broiler chickens of the Arbor Acres cross. In our research we used: probiotic based on Bacillus cereus strain and coumarin. Experimental scheme: control group (C) — the main diet (MD), experimental group I — MD + Bacillus cereus (dose of 12.6˟103 microbial bodies/kg of feed/ day), experimental group II — MD + coumarin (dose of 2 mg/kg of feed/day), experimental group III — MD + Bacillus cereus + coumarin. The experiment period is 35 days. Analysis of the elemental composition of tissues was determined by inductively coupled plasma mass spectrometry and inductively coupled plasma atomic emission spectrometry on a "Nexion 300D" quadrupole mass spectrometer and an "Optima 2000 DV" atomic emission spectrometer.Results. As a result of experiments conducted on feeding probiotics to broilers based on Bacillus cereus and coumarin strains, their positive effect on the accumulation of essential and conditionally essential trace elements in the liver and pectoral muscles and a decrease in the presence of a number of toxic elements was established.