Study of the Fermentation Conditions for 5-Aminolevulinic Acid Production by <i>Rhodopseudomonas palustris</i>

Abstract

Culture conditions for Rhodopseudomonas palustris RB to produce 5-Aminolevulinic Acid (ALA) was investigated. As results, the best culture conditions for accumulation ALA were 10 % inoculating concentration, light intensity 3000 Lux and its pH value was 7.0. The production of ALA can be improved by useful of resisting the activity of ALA dehydratase in the biosynthetic pathway. And the experimental results showed that Gly was the key precursor substance for ALA. Under the optimal conditions, the production of ALA by this strain was as high as 9.0 mg/L when 45 mM LA and 60 mM were implanted, which was 2 times higher than the control.