Study of the effect of some drugs used in the clinic on the AQP1 activity of the human erythrocyte membrane

K V Basiakova,E P Titovets

doi:10.29235/1814-6023-2021-18-2-204-211

Abstract

Using the stopped flow method and based on the study of the intensity of light scattering, the effect of pharmacological preparations used in the clinic on the water exchange of human erythrocytes, catalyzed by aquaporin AQP1, was studied. Pharmacological preparations used in therapeutic concentrations have a variable inhibitory effect on water permeability of the erythrocyte membrane. The obtained results broaden our understanding of the molecular action mechanism of the investigated drugs. In view of the wide distribution of AQP1 in various human tissues, these data should be taken into account when carrying out therapeutic measures aimed at normalizing the water exchange of organs and tissues.

Highlights

Using the stopped flow method and based on the study of the intensity of light scattering, the effect of pharmacological preparations used in the clinic on the water exchange of human erythrocytes, catalyzed by aquaporin AQP1, was studied
Pharmacological preparations used in therapeutic concentrations have a variable inhibitory effect on water permeability of the erythrocyte membrane
The obtained results broaden our understanding of the molecular action mechanism of the investigated drugs

Summary

Introduction

Using the stopped flow method and based on the study of the intensity of light scattering, the effect of pharmacological preparations used in the clinic on the water exchange of human erythrocytes, catalyzed by aquaporin AQP1, was studied. ИССЛЕДОВАНИЕ ДЕЙСТВИЯ НЕКОТОРЫХ ПРЕПАРАТОВ, ПРИМЕНЯЕМЫХ В КЛИНИКЕ, НА АКТИВНОСТЬ AQP1 МЕМБРАНЫ С использованием метода остановленного потока и на основе исследования интенсивности светорассеяния изучено действие применяемых в клинике фармакологических препаратов на водный обмен эритроцитов человека, катализируемый аквапорином AQP1. Установлено, что примененные в терапевтических концентрациях лекарственные средства оказывают разной степени выраженности ингибирующее влияние на водную проницаемость мембраны эритроцитов.

Results

Conclusion