Study of the Antiviral Activity and Toxicity of Dextrin - In Vitro Study

Abstract

Objective: This study was to in vitro study of the antiviral effect of "Dextrin" on the replication of the EMCV virus in HeLa cells and toxicity of "Dextrin". Methods: For this purpose, the virus at a dose of 100 TCD50 is applied to a 24-hour monolayer of HeLa cells. The experiment is carried out under conditions of 5% CO2 saturation and 100% humidity. The virus dose used was expressed as a tissue cytopathic dose of 100 TCD50/ml. Cultivation of HeLa cells, to study toxicity, was performed on 96-well plates under conditions of 5% CO2 saturation and 100% humidity. The toxic effect of "Dextrin" on HeLa cells was studied by the degree of destruction of the monolayer and at the population level. Results: Peak levels of the virus reached after 48 hours of replication accompanied by complete destruction of the cell monolayer (7-8 cycles of replication). As follows from the graph, “Dextrin significantly” (significance is indicated by asterisks.) reduces the dose of the virus when introduced into the medium with HeLa cells to, or at the early stages of EMCV virus replication (-1,0 and 1 hour). Based on the data obtained, it was decided to use a dose of 1:100 (1%), which is the highest non-toxic dose. Conclusion: “The investigated Dextrin has an antiviral effect against the EMCV virus, when applied in the early stages of infection. This may open up the prospect of studying the antiviral spectrum of Dextrin in more future studies”.