Study of the Antioxidant Effect of <italic>Ojayeonjong-hwan</italic>

Abstract

Objective: In this study, the antioxidant activity of <i>Ojayeonjong-hwan</i> extracts was compared, and the following results were obtained.Methods: For hydrothermal and ethanol extracts, DPPH free radical and ABTS cationic radical erasing activity and reducing power using the FRAP method were compared, and the association between the antioxidant power of each extract and total phenol content was investigated. Significant results were obtained through in vitro apoptosis analysis through FFITC staining, mitochondrial membrane potential analysis, and ROS level measurement using C2C12 myoblastoma.Results: 1. In a comparison of DPPH free radical and ABTS cationic radical scavenging activity, water, and 70% ethanol extracts of <i>Ojayeonjong-hwan</i> (WEO and EEO) showed superior radical scavenging ability. <br/>2. In the results of reducing power using the FRAP method, WEO and EEO showed antioxidant activity, which was shown to be dependent on the total phenol content contained in the extracts. <br/>3. In comparison to the protective effect against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in C2C12 myoblasts, water extracts had no significant effect, but 70% ethanol extracts inhibited H<sub>2</sub>O<sub>2</sub>-mediated cytotoxicity in a concentration-dependent manner. <br/>4. The cytotoxic protective effect of EEO against oxidative stress in C2C12 myoblasts was correlated with its inhibitory effects on H<sub>2</sub>O<sub>2</sub>-induced apoptosis and cell-cycle arrest. <br/>5. In H<sub>2</sub>O<sub>2</sub>-treated C2C12 myoblasts, the apoptosis inhibitory effects of EEO were associated with the suppression of mitochondrial dysfunction and DNA damage. <br/>6. The protective effects of EEO against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in C2C12 myoblasts were directly related to the inhibition of ROS generation.Conclusions: <i>Ojayeonjong-hwan</i> extracts all have protective potential against oxidative stress.