Study of the action of nanostructured sapropel at different doses on the morphological and functional condition of the gastrointestinal tract in albino mice

Abstract

A nanostructured sapropel with a particle size of 45.0–180.0 nm has been manufactured by ultrasonic impact on sapropel. While studying its acute oral toxicity in albino mice, a safe dose of 0.3 g/kg of body weight is determined. A single intragastric administration at doses of 1.2, 1.8, and 2.4 g/kg of body weight reveals a dose-dependent character of intoxication symptoms. A dose of 3.0 g/kg of body weight is sublethal. During a diagnostic autopsy of mice treated with nanostructured sapropel at a dose of 0.3 g/kg, we observed the enhancement of physiological processes of the secretory activity in the gastrointestinal tract. Histological examination shows a moderate secretion of mucus and the integrity of the surface epithelial cells of the esophagus, stomach, and duodenum. Acute catarrhal inflammation of the esophagus, stomach, and duodenum is observed in mice that receive the preparation at a dose of 1.8 g/kg. A focal deformation of the surface epithelial cells with the preservation of their cytology and adhesion of the sapropel conglomerates by mucus are revealed by microscopic examination. Catarrhal-hemorrhagic esophagitis, gastritis, and duodenitis are observed in mice treated with the nanostructured sapropel at a dose of 3.0 g/kg. During a histological examination of the gastrointestinal tract in the areas of contact with nanostructured sapropel, thinning and disruption of mucosal integrity, desquamation, and lysis of epithelial cells are found.