Abstract
In the present work a novel enzyme based biosensor has been developed by immobilizing glucose oxidase onto ITO electrode modified with biocompatible composite consisting of chitosan/polypyrrole-nanotubes and gold nanoparticles nanocomposites. Polypyrrole nanotubes and gold nanoparticles nanocomposites have been synthesized by self degradation method followed by in-situ reduction of auric chloride. The formation of PPy nanotubes and uniform decoration of Au nanoparticles over the nanotubes have been confirmed by HRTEM studies and SEM (Scanning Electron Microscopy). XRD results confirm the formation of PPy-NTs/Au-NPs nanocomposites. The enzyme electrode exhibited a well defined redox peak with formal potential (E0) of −0.45V. The response of modified electrode showed a quasi reversible behaviour and a surface confined electrode process. A response time of less than 40s has been found for glucose determination using the modified electrode. The bioelectrode depicted good linearity of 3–230μM (R2=0.98) towards the oxidation of glucose with a sensitivity of ca. 149μAμM−1mL and detection limit of 3.10μM. The calculated value of heterogeneous electron transfer rate constant (ks) was determined to be 2.54s−1. The pH dependence of E0 suggests that the direct electron transfer of GOx was a two electron and two proton transfer reaction process.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
