Abstract

The current study deal with 214 clinical samples taken from in- and outpatient's of both sexes with different ages of Al-Hilla Teaching Hospital, Babylon Maternity and Pediatrics Hospital in Hilla city. All tests were conducted in the laboratories of the University of Kufa, College of Science, Department of Ecological and Pollution Sciences, in 2022. 57 bacterial isolates (26.6%) were isolated and identified according to their phenotypic characteristics and biochemical tests. The current study declared that the isolation percentage was 39 (18.2%) and 18 (8.4%) for S. aureus and S.epidermidis respectively. The antibiotics sensitivity to isolates were tested, as it included 27 antibiotics, by disk diffusion method, most of it showed high resistance. Meropenem, ceftriaxone, and amikacin were found to be the most effective antibiotics, the phytosynthesized ZnO (NPs) were also tested on the sensitivity of the isolates. The lowest inhibitory concentrations (MIC) for current isolates were high. While the ZnO (NPs) showed higher efficiency and lower concentrations. This study is important in pharmacological and epidemiological studies and could open up broader horizons to find alternatives to the antibiotics used previously.

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