Abstract

Disposable diapers are one of many possible sources of infectious enteric viruses that are disposed of in landfills. A total of 218 disposable diapers were collected from 7 sites and 10 depths at three landfills. Of this total, 110 diapers were selected be processed based on fecal content using a 1.5% beef extract elution, organic flocculation-concentration method to recover viruses. The concentrated samples were assayed on Buffalo Green Monkey (BGM) Kidney cell cultures for the detection of enteroviruses and with cDNA probes specific for poliovirus, hepatitis A virus, and rotavirus. Enteroviruses were not detected in any sample assayed using cell culture techniques

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