Coxsackievirus B-3 selection of virus resistant Buffalo green monkey kidney cells and chromosome analysis of parental and resistant cells.

Abstract

Following coxsackievirus B-3 (CBV-3) infection and lysis of highly susceptible Buffalo green monkey kidney (BGMK) cells, there was a regrowth of cells. Cultures of regrown cells were established and they continued to release infectious CBV-3 for up to 20 weeks. The parental BGMK cells were susceptible to CBV-3, CBV-4 and poliovirus type 2 induced cytopathic effect (CPE), while the cured cells were resistant to CBV-3 and CBV-4 but not to poliovirus type 2. Attachment of CBV-3 was restricted on cured BGMK cells but not on parental cells. Chromosome analysis showed that the cured cells originated from the BGMK cell line and that they were missing two marker chromosomes present in the parental cells.