Abstract
A procedure for the combined purification of both indolyl-3-acetic acid (IAA) and abscisic acid (ABA) is presented. The procedure is designed to obtain accurate quantifications with minimal losses and within a relatively short time. The prepurification procedure makes optimal use of CI 8 prepacked cartridges. A purification step by high pressure liquid chromatography is included and discussed. The quantification of IAA is performed with the 2-methylindolepyrone assay (2-MIP-assay). An alternative way of correcting for aspecific fluorescence is proposed and tested. ABA is analysed by GLC-ECD. The accuracy of the overall method is tested using different approaches for both ABA and IAA. Using this method, extraction by homogenization is compared to extraction by diffusion without homogenization.
Published Version
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