Study of NFKB Polymorphism with HPV Infection in Women of Reproductive Age Group

Abstract

Background: Human Papilloma Virus (HPV) is highly prevalent, with around 80% of women thought to be infected at some point. They are thought to produce proteins which inhibit the tumour suppressor protein p53 in cervical epithelial cells, allowing for uncontrolled cell division. The persistent HPV infection changes a premalignant lesion to cancerous state and this seems to be accompanied by a progressive loss of responsiveness to the NFƙB mediated growth inhibitory signal. This study was undertaken to provide a more precise evaluation of association of NFƙB -94ins/del ATTG promoter polymorphism and cancer risk. Methods: The study included 75 cases (unhealthy cervical pathology) and 75 controls (healthy normal cervix) belonging to reproductive age (15-44years) group. Cervical swab for HPV genotyping was performed in cobas x 480 and cobas z 480 analysers. DNA was extracted from EDTA blood sample using cobas DNA sample preparation kit. NFƙB gene polymorphism was seen by PCR-RFLP method in gel electrophoresis. Statistical analysis was done using SPSS 20. Results: Among the healthy normal cervical cytology, HPV infection rate was found to be 6.6%. In cases, maximum HPV positivity was for HPV 16. Among the study population, insertion allele was seen more in both the study groups compared to deletion allele. We found del/del genotype contribution maximum among the cases with cervical pathology. Ins/del genotype was found in cases group. In unhealthy cervix, we found that ins/ins genotype was prevalent among all the HPV strain except HPV 18 followed by del/del genotype. But, among HPV 18 strain, del/del was seen more common.